Team: Purdue/Enzymes

• INTRODUCTION
• BACKGROUND
  • The Problem
  • Organisms
  • Enzymes
• PROJECT
  • Description
  • Project Development
  • Our Solution
• PARTS
  • Team Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
  • Parts Used
  • Parts Submitted
• NOTEBOOK
• ATTRIBUTIONS
• OUR PROCESS
• HUMAN PRACTICES
• SAFETY
• MODELING

Enzymes

Lignin Peroxidase

Suraj's words here

Versatile Peroxidase

Erich's word here

Manganese Peroxidase

Suraj's words here

Laccase

Erich's words here

Tyrosinase

Erich's words here

Aldo-Keto Reductase

Aldo-Keto Reductase

Aldo-Keto Reductases are a family of enzymes with NADPH dependent oxidoreductases. Oxidoreductases work in the transfer of electrons, moving electrons from one molecule to another, or from reductants to oxidants. AKRs are expressed in all plants and animals. They play a role in the human body working to detoxify pharmaceuticals and drugs in the liver. Aldo-keto reductases (AKRs) have also been found to be highly expressed in the termite gut. A similar mechanism occurs in the termite gut, where toxins released from the breakdown of lignin are mediated by AKRs. AKRs have a beta-alpha-beta fold which binds via a NADP-binding motif. According to Dr. Michael Scharf, these aldo-keto reductases work as helper enzymes that exponentially aid in the digestion and breakdown of lignin, but are not as effective on their own.

pH Range (including optimal): 4.5-9.5 Optimal: 7

Temperature Range (including optimal): 0-55 C Optimal: 25-37

Cellulase

Cellulase is an enzyme that plays a large role in the decomposition of cellulose. Specifically this enzyme works on the hydrolysis of the beta glycosidic linkages, precisely 1,4-beta-D-glycosidic linkages, in cellulose. Cellulase is not present in the human body, therefore the cellulose present in plant materials that are consumed never become broken down and are passed through the body. The main function of cellulase is to break down cellulose into monosaccharides or oligosaccharides that can be used in several ways. Cellulase is often used to make the raw materials in plants soluble for use in products like juices, alcoholic beverages, feed additives, and washing agents.

Xylanase

Jill's words here