Teams:Lethbridge/Measurement
Meausurement
Our team decided to take part in this year’s interlab measurement study, measuring fluorescence of GFP in devices 1, 2 and 3. The GFP sequence was cleaved from I13504, using restriction enzymes Xba1 and Pst1, and ligated into pSB1C3 plasmid containing the following parts J23101, J23106 and J23117-cleaved with Spe1 and Pst1. Sequencing and restriction mapping was used to confirm proper insertion of the GFP sequence. Devices were transformed into DH5α cells. DH5α cells containing the devices and controls were normalized, lysed and GFP measurements were taken using a fluorimeter.
