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We established a NEW APPROACH to work with all types of functional nucleic acids, setting the foundations for their widespread use in synthetic biology

• We developed a NEW STANDARD to easily clone functional RNAs for their expression in vitro and in vivo (RFC 110)

• We established a NOVEL dual-color readout system to monitor the in vitro transcription of an RNA of interest based on a new ATP-switchable Spinach II aptamer

• We established a NOVEL detection method for short ssDNAs and ssRNAs based on the HRP-mimicking DNAzyme

• We designed de novo an ACTIVE SITE for twin ribozyme and proved its efficient cleavage activity in living yeast cells

• We conceived and implemented an open-source software for the design of aptamers (MAWS) as a fast and affordable alternative to the laborious SELEX procedure

• We conceived and implemented an open-source software for the design of aptazymes (JAWS) to enable the construction of new sensing devices

• We EXPERIMENTALLY VALIDATED several of our designed aptamers and aptazymes and fed the collected data back into the algorithms

We proved the PRINCIPLE that a ketamine-switchable aptazyme could be used to implement a TEST STRIPE to detect RAPE DRUGS in situ

We established a NEW APPROACH to work with all types of functional nucleic acids, setting the foundations for their widespread use in synthetic biology