Difference between revisions of "Template:Team:Groningen/CONTENT/EXPERIMENTS/Restriction analyse (th8)"

Latest revision as of 23:43, 21 November 2015

00:00, 28 Augustus 2015 - 00:00, 28 Augustus 2015

A restriction analysis of the miniprepped thrC locus 1 was performed. To 5 µL from each plasmid, 13 µL MiliQ, 1 µL fast digest EcoRV and 1 µL fast digest buffer was added. The mixtures were incubated for one hour at 37 °C. The correct plasmid would have 2 bands; K823023 (original) or pdg1664 (original) would show one band. Fortunately, all 5 plasmids had two bands. One of the plasmids will be used further (thrC plasmid A).

Marieke

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-Restriction analyse miniprep thrC locus 1
+A restriction analysis of the miniprepped thrC locus 1 was performed. To 5 µL from each plasmid, 13 µL MiliQ, 1 µL fast digest EcoRV and 1 µL fast digest buffer was added. The mixtures were incubated for one hour at 37 °C. The correct plasmid would have 2 bands; K823023 (original) or pdg1664 (original) would show one band. Fortunately, all 5 plasmids had two bands. One of the plasmids will be used further (thrC plasmid A).
-To 5 ul from each plasmid was added:
-ul MiliQ
-ul fast digest EcoRV
-ul fast digest buffer
-incubated for 1 hour at 37C
-Good plasmid would have 2 bands
-K823023 (original) or pdg1664 (original) would have 1 band.
-All 5 plasmids had two bands. One of the plasmids will be used further.
-named as thrC plasmid A
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