A restriction analysis of the miniprepped thrC locus 1 was performed. To 5 µL from each plasmid, 13 µL MiliQ, 1 µL fast digest EcoRV and 1 µL fast digest buffer was added. The mixtures were incubated for one hour at 37 °C. The correct plasmid would have 2 bands; K823023 (original) or pdg1664 (original) would show one band. Fortunately, all 5 plasmids had two bands. One of the plasmids will be used further (thrC plasmid A).
Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Restriction Analysis th8"
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|protocol=none | |protocol=none | ||
|protocolurl=none | |protocolurl=none | ||
| − | |goal= | + | |goal=Extra integration of DNA in thrC locus |
| − | |description= Restricion analysis from plasmids | + | |description=Restricion analysis from plasmids |
|conclusion=Correct analysis from the plasmids | |conclusion=Correct analysis from the plasmids | ||
}} | }} | ||
Latest revision as of 23:44, 21 November 2015
Restriction analyse (th8)
Restricion analysis from plasmids
Extra integration of DNA in thrC locus
Correct analysis from the plasmids
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/none">none</a>
00:00, 28 Augustus 2015 - 00:00, 28 Augustus 2015