Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Restriction th11"
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Latest revision as of 23:46, 21 November 2015
Restriction K823023 (th11)
Digestion of K823023
Extra integration of DNA in thrC locus
K823023 was digested
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
K823023 was digested with EcoRI and AatII for 2 hours at 37 °C. The restriction samples were run on a 1% agarose gel and the correct bands were cut out of the gel and purified with the PCR purification kit from Thermo Scientific.