Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Restriction with Inserts th21"
| Line 1: | Line 1: | ||
{{Team:Groningen/TEMPLATES/EXPERIMENT | {{Team:Groningen/TEMPLATES/EXPERIMENT | ||
| − | |title=Restriction thrC (th21) | + | |title=Restriction thrC compleet, tasA, bslA and slrR Inserts (th21) |
|protocol=Restriction | |protocol=Restriction | ||
|protocolurl=restriction | |protocolurl=restriction | ||
|goal=Extra integration of DNA in thrC locus | |goal=Extra integration of DNA in thrC locus | ||
| − | |description=Digestion of thrC | + | |description=Digestion of thrC completed |
|conclusion=Digested products | |conclusion=Digested products | ||
}} | }} | ||
Latest revision as of 23:23, 21 November 2015
Restriction thrC compleet, tasA, bslA and slrR Inserts (th21)
Digestion of thrC completed
Extra integration of DNA in thrC locus
Digested products
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 6 September 2015 - 00:00, 6 September 2015
The following components were used.
Construct
Restriction enzymes
ThrC backbone
EcoRI and PstI
Restriction.
The restriction was performed for 2 hours at 37 °C in stopped by heating at 80°C for 20 min.