Difference between revisions of "Troubleshooting/Ligation"
| Line 12: | Line 12: | ||
<a href= "https://2015.igem.org/Troubleshooting#tips"> <li>General Tips and Tricks</li></a> | <a href= "https://2015.igem.org/Troubleshooting#tips"> <li>General Tips and Tricks</li></a> | ||
<ol><a href= "https://2015.igem.org/Troubleshooting/Transformation"> <li>Transformation</li></a> | <ol><a href= "https://2015.igem.org/Troubleshooting/Transformation"> <li>Transformation</li></a> | ||
| − | <a href="https://2015.igem.org/Troubleshooting/Ligation"> <li>Ligation</li></ol> | + | <a href="https://2015.igem.org/Troubleshooting/Ligation"> <li>Ligation</li> |
| + | <a href="https://2015.igem.org/Troubleshooting/Restriction"> <li>Restriction Digest</li></ol> | ||
<a href= "https://2015.igem.org/Troubleshooting#protocols"> <li>iGEM Protocols</li></a> | <a href= "https://2015.igem.org/Troubleshooting#protocols"> <li>iGEM Protocols</li></a> | ||
<a href= "https://2015.igem.org/Troubleshooting#answer"> <li>Answers</li></a> | <a href= "https://2015.igem.org/Troubleshooting#answer"> <li>Answers</li></a> | ||
Revision as of 18:39, 7 May 2015
Ligation Troubleshooting
Now that you know you transformation efficiency and it's above 1 x 108 CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.
No colonies