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| − | <h4><a href= "https://2015.igem.org/Troubleshooting">Troubleshooting</a></h4>
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| − | <ul>
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| − | <a href= "https://2015.igem.org/Troubleshooting#intro"> <li>Introduction</li></a>
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| − | <a href= "https://2015.igem.org/Troubleshooting#tips"> <li>General Tips and Tricks</li></a>
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| − | <ol><a href= "https://2015.igem.org/Troubleshooting/Transformation"> <li>Transformation</li></a>
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| − | <a href="https://2015.igem.org/Troubleshooting/Ligation"> <li>Ligation and Restriction Digest</li></ol>
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| − | <a href= "https://2015.igem.org/Troubleshooting#protocols"> <li>iGEM Protocols</li></a>
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| − | <a href= "https://2015.igem.org/Troubleshooting#answer"> <li>Answers</li></a>
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| − | <a href= "https://2015.igem.org/Troubleshooting#ask"> <li>Ask Your Questions</li></a>
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| − | <div class="alertMessage"> Page is under construction. <br>iGEM HQ is currently working on updating this information
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| − | for the iGEM 2015 competition. </div>
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| − | <h2>Ligation and Restriction Digest Troubleshooting</h2>
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| − | Now that you know you <a href"https://2015.igem.org/Troubleshooting/Transformation">transformation efficiency</a> and it's above 1 x 10<sup>8</sup> CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations and restriction digests.
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| − | <br><br>
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| − | <b>No colonies</b>
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| − | If you know your cells are working well, there are a few common ligation problems that might be happening with your reaction.
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| − | <ol><li><b>Uncut insert:</b> It's possible that your insert was not cut well during your digest. </li>
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| − | <li><b>Ligase didn't work:</b>
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| − | </html> {{2015Bottom}}<html>
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| − | </html>
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