Troubleshooting/Ligation

Troubleshooting

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1. Transformation
2. Ligation
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Ligation Troubleshooting

Now that you know you transformation efficiency and it's above 1 x 10⁸ CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning.

Here are some tips to help you troubleshoot this problem:

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