Troubleshooting/Ligation

Troubleshooting

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1. Transformation
2. Ligation
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Ligation Troubleshooting

Now that you know you transformation efficiency and it's above 1 x 10⁸ CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.

Here are some tips to help you troubleshoot this problem:

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