Demo informationpage
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based on standard parts
Team Information - Columbia NYC
- Stanford University Online High School, CA, USA ohs.stanford.edu
- Hankuk Academy of Foreign Studies Kyunggi, Korea www.hafs.hs.kr
- Cheongshim International Academy, Kyunggi, Korea http://www.csia.hs.kr/
- ✔ Your registration fee has been received
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- ✔ Resource description has been submitted
- Section: ? Undergraduate
- Region: Europe
- Application Date: 2015-02-21
- Acceptance Date: 2015-04-13 14:21:14
- ✔ 10 Check In Form
- ! 10 Visa Application Day
- ! 19 InterLab Measurement study sign up
- ✔ 22 About our Lab form
- ✔ 25 About our Project form
- ✘ 15 About our Project form
- ✘ 15 Project descriptiondue
- ✘ 07 Track selection due
- ✘ 07 Title and abstractdue
- ✔ 28 Final Safety form
- ! 10 Team banners due
- ✔ 11 Team rostersdue
- ✘ 18 Part submission send by date
- ✘ 18 Judging form due
- ! 18 Wiki freeze
- ✔ 11 Keywordsdue
- ! 31 Wiki freeze archive
New Application
- New Application
- Manufacturing
- Measurement
BBa_K1606000
to
BBa_K1606999
- Insects
- toolkit
- Spodoptera frugiperda
The purpose of this project is to introduce the Sf9 cell line (Fall armyworm ovary, Spodoptera frugiperda cells) to synthetic biology and the iGEM Competition, by developing a tool kit of several biobricks for their use in this new chassis. We will use the Sf9 cells as an alternative for the production of proteins with high complexity and post-traductional modifications, because of their flexibility, cultivation time and minor cost compared with other expression systems. We will characterize functional parts for two main areas: Transfection and Genome Editing. For the first part we will characterize baculovirus mediated transfection and, direct plasmid transfection for the generation of stable cell lines; for the second part we will use the CRISPR/Cas9 system to assay highly specific genome modifications in an effort to improve production and ease of use of this technology.
Gold
Best Manufacturing Project, Overgrad
Best Manufacturing Project, Overgrad
Best Software Tool, Overgrad
Best Part Collection, Overgrad