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4 KB (529 words) - 01:24, 19 September 2015
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- ...s, the expression of the three plasmid system in the control group without Ack has the same level. While the expression of the three plasmid system is exp ...le system has been improved. In addition, the average expression of adding Ack experimental group has reached 790.5, and in others successful transfection22 KB (2,875 words) - 02:42, 19 September 2015
- ...cy of our orthogonal system, we devided the cells into two groups. One has Ack in the culture medium and another does not. ...gene in the circuits can be selectively expressed in Experiment group with Ack. As shown in Fig. 1, the activity of Renilla Luciferase(RLUC) varied widely7 KB (893 words) - 23:59, 18 September 2015
- ...cy of our orthogonal system, we devided the cells into two groups. One has Ack in the culture medium and another does not. ...gene in the circuits can be selectively expressed in Experiment group with Ack. As shown in Fig. 1, the activity of Renilla Luciferase(RLUC) varied widely6 KB (937 words) - 13:06, 18 September 2015
- <h4>2.The UAA using in our system:Acetyllysine(ACK)</h4> <p>Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl16 KB (2,140 words) - 23:02, 18 September 2015
- <h4>The UAA using in our system:Acetyllysine(ACK)</h4> <p>Acetyllysine(ACK) is one of unnatural amino acid and only corresponds to a special aminoacyl7 KB (1,005 words) - 06:26, 18 September 2015
- ...-transferase enzyme that converts acetyl-CoA into acetyl-phosphate, and <i>ack</i>, encoding for an acetate kinase that removes acetyl-phosphate's phospha ...'s acetate production (only <i>acs</i> was knocked out for this strain, <i>ack</i> and <i>pta</i> were not yet overexpressed), we conducted the turbidosta13 KB (1,824 words) - 01:58, 19 September 2015
- #ack-1{ #ack-2{342 B (32 words) - 08:40, 16 September 2015
- ...nce it only demonstrated significant activity in bladder cancer cells with Ack.</p>10 KB (1,347 words) - 22:00, 18 September 2015
- ...d, AckRS can produce an RNA synthetase which can achieve the attachment of Ack and tRNA. This composite part will perform its function together with two o8 KB (1,117 words) - 22:26, 18 September 2015
- <p>The Pta1, Pta2, Δacs and Ack strains were cultured on photostats. This system, developed by <a href = "h14 KB (2,050 words) - 20:18, 2 October 2015
- ...Aminoacyl tRNA carrying anticodon CUA, can recogniaze codon UAG and incert Ack into peptide </td>13 KB (1,453 words) - 23:02, 18 September 2015