Team:Groningen/Notebook/Backbone Groningen
Blue Bio Energy
Backbone from previous iGEM Groningen team
Growing a backbone from a previous iGEM Groningen team.
To grow a backbone.
Partial success.
None
00:00, 30 April 2015 - 00:00, 30 April 2015
Grew 3 different possible backbones overnight in LB + AMP and LB + CM
00:00, 1 June 2015 - 00:00, 1 June 2015
1 grew in AMP, 2 and 3 grew in CM.
1 is E. coli and the other two are B. subtilis and have no plasmids.
Miniprep culture #1 (Thermo Scientific #K0502, lot: 00272723)
stored as: ‘RFP backbone’ in -20 °C (50 µL, 90 ng/µL DNA).