Team:Groningen/Notebook/Ligation Backbone pg14

Blue Bio Energy
Ligation Backbone (pg14)
Ligate the combined part of PGA into pSB1C3 to get the DNA into a backbone.
Make a more negatively charged biofilm.
The ligation did not work according to the following colony PCRs
Ligation
12:00, 22 July 2015 - 12:00, 22 July 2015
Ligation of PGA into pSB1C3 according to protocol.
Bente Bouwmeester