Team:Groningen/Notebook/Restriction analysis yuab
Blue Bio Energy
Restriction analysis yuaB (BslA) en TasA
Restriction analysis was performed. Results were analysed using Gel electrophoresis.
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None
00:00, 10 Juli 2015 - 00:00, 10 Juli 2015
This experiment consists of four phases:
Restriction
PCR
Gel electrophoresis
Analysis of the gel
Four samples of yuaB and four samples of tasA were analyzed.
Substance
Quantity
plasmid yuaB/TasA
6 uL
2.1 buffer
2 uL
PstI
1 uL
EcoRI
1 uL
H2O
10 uL
Mix for restriction.
Restriction was performed according to page 14 of the protocol book. After PCR gel electropheresis was performed using a gel doc XR to check if there are bands of the correct size. Sample 4 of yuaB and sample 2 of TasA seemed correct but sequencing proved that they were not.