Team:Groningen/Notebook/tasA Primer design t1
Blue Bio Energy
![](https://static.igem.org/mediawiki/2015/f/f5/Igem.groningen.2015.show.details.transparent.png)
tasA Primer Design t1
Design primers for the isolation of DNA and getting rid of restriction sites within the gene.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
Designing the primers was successful.
None
00:00, 16 April 2015 - 00:00, 16 April 2015
Primers were designed for tasA in B. subtilis 168.
#
Primer
Sequence
5
tasA for
CGGAATTCGCGGCCGCTTCTAGAGCAATAAAAGGGGAGCTTACCATG
6
tasA rev
CGCTGCAGCGGCCGCTACTAGTATTATTAATTTTTATCCTCGCTATGC
7
tasA EcoRI overlap for
GATTTATCTGCTAAAGAGAACTCAGCGAGTGTGAAC
8
tasA EcoRI overlap rev
GTTCACACTCGCTGAGTTCTCTTTAGCAGATAAATC
9
tasA PstI overlap for
GAGTTTAGGAGTTGCTTCAGCAGCACTAGGATTAG
10
tasA PstI overlap rev
CTAATCCTAGTGCTGCTGAAGCAACTCCTAAACTC
Primers for tasA in B. subtilis 168.
![](https://static.igem.org/mediawiki/2015/6/62/Igem.groningen.2015.figure.large.primerstasA_0001.png)
Primers for tasA.