Team:Paris Saclay/Notebook/August/24

Monday 24th August

Lab Work

New culture

by Audrey

  • BBa_K1707037 #1 to #10

Electrophoresis

by Audrey

  • BBa_K1707004 #5 (digested by SpeI + EcoRI)
  • BBa_K1707022 (digested by XbaI + PstI)

Agarose gel 1%, Migration 90V

We don't have any gel picture because the gel broked. We should do this electrophoresis again.

Digestion

by Audrey

  • BBa_K1707004 #5 and BBa_K1707023 #1:
    • 10 µL plasmid
    • 1 µL SpeI
    • 1 µL EcoRI
    • 2 µL Buffer 2 NEB
    • 6 µL H2O
  • BBa_K1707004 #5 and BBa_K1707023 #1:
    • 10 µL plasmid
    • 1 µL SpeI
    • 1 µL PstI
    • 2 µL Buffer 2 NEB
    • 6 µL H2O
  • BBa_K1707022 #1:
    • 15 µL plasmid
    • 1 µL XbaI
    • 1 µL PstI
    • 2 µL Buffer FastDigest 10x
    • 1 µL H2O
  • BBa_R0040:
    • 20 µL plasmid
    • 1 µL XbaI
    • 1 µL EcoRI
    • 3 µL Buffer FasrDigest 10x
    • 5 µL H2O

Incubation 1h30, 37°C

Member present:

  • Instructors: Claire
  • Students: Audrey

Back to the calendar

Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.