Wednesday 15th July

Lab Work

Observation of our plates

by Coralie and Johan

Transformations from the 07/10/2015

We can observe that BBa_S03518, BBa_B0030 and BBa_B0015 are OK. But BBa_K1399005 didn't grow. We think that we make a mistake when we rehydrated the plasmid.

Transformations from the 07/09/2015

We can observe that BBa_J23101 + BBa_I13504 and BBa_J23106 + BBa_I13504 are yellow when we expose them to the white light. But BBa_J23117 + BBa_I13504 don't seem to be yellow on the white light.

New culture

by Coralie, Johan and Seong Koo

New liquid culture of:

Transformations from the 07/09/2015:
- BBa_J23101 + BBa_I13504
- BBa_J23106 + BBa_I13504
- BBa_J23117 + BBa_I13504

BBa_B0015
BBa_B0030
BBa_S03518

2x 5ml LB + 10μl Chloramphenicol + 1 bacterial colony. We incubate cultures at 37°C, ON.

Ligation

by Pauline

Reaction mix:

BBa_J23101: 1µL
BBa_K115017: 10µL
Buffer (10x): 2µL
Ligase: 1µL
H2O: 6µL

Incubation 3h at 4°C

Transformation

by Seong Koo

BBa_K139905
BBa_K1399019
BBa_K1399023

Ligation product:

BBa_J23101 + BBa_K115017 = BBa_K1707000

Plasmid extraction

BBa_R0051

Culture form the 07/10/2015 We put back in culture 200µL of this one in 5mL of LB + Ampicilline

With the Nucleospin Kit from Macherey Nagel

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Coralie, Pauline

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Team:Paris Saclay/Notebook/July/15

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