Thursday 9th July

Lab Work

Transformation

by Coralie

Ligation product:

BBa_J23101 + BBa_I13504
BBa_J23106 + BBa_I13504
BBa_J23117 + BBa_I13504

On LB + Chloramphenicol 20ug/mL. Incubation ON, 37°C

by Johan and Seong Ko

BBa_R0051

On LB + Ampicillin 100ug/mL. Incubation ON, 37°C

Plasmid Rehydratation

by Johan and Audrey

BBa_S03518
BBa_B0030
BBa_B0015
BBa_K1399005

Digestion

by Pauline and Audrey

Plasmid with promotor: BBa_J23101

10µL of our plasmid with promotor
1µL SpeI
1µL PstI
2µL buffer 10x FD

Plasmid with gene: BBa_COO40 and BBa_K115017

10µL of our plasmid with gene
1µL XbaI
1µL PstI
2µL buffer 10x FD

After this step, we separate the sequence we need from the sequence we don't with electrophoresis

Electrophoresis

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V

Quantification, from left to right: 1. DNA Ladder, 2. BBa_C0040, 3. BBa_K115017, 4. BBa_J23101, 5. Empty, 6. Empty, 7. Empty, 8. Empty, 9. Empty, 10. Empty, 11. Empty, 12. Empty

Quantification

by Pauline and Audrey

Preparation of Agarose Gel 1%, 0,5g in 50mL of 1X TAE, 0,5µL of BET Migration 0,06A 80V We quantify with the electrophoresis gel:

BBa_C0040: 10ng/µL
BBa_J23101: 20ng/µL
BBa_K115017: we don't see enough of fluorescent to quantify the biobrick. We decide to amplify this one by PCR.

Members present:

Instructors and advisors: Alice.
Students: Johan, Seong Koo, Audrey, Coralie, Pauline

Back to the calendar

Team:Paris Saclay/Notebook/July/9

Contents

Thursday 9th July

Lab Work

Transformation

Plasmid Rehydratation

Digestion

Electrophoresis

Quantification