Team:TU Darmstadt/Notebook/sec1/K1602026
K1602026 - T7-B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (T7-B0034-His-GFP-Si4)
K1602025 was digested with XbaI and PstI and ligated into T7-pSB1A2. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened using a colony PCR with VF2 and VR (Figure 1).
![](https://static.igem.org/mediawiki/2015/0/08/Figure_4-_Colony_PCR_of_T7-B0034-Prefix-His-Tag-GFP-Si4.png)
Figure 1 Colony PCR of T7-B0034-His-GFP-Si4. All colonies (1-3) contained the insert of around 1.1 kbp. DNA marker: 2-Log DNA Ladder.