Team:TU Darmstadt/Notebook/sec1/K1602029

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K1602029 - T7-B0034-HistidinTag-Scaffold (T7-B0034-His-Scaffold)

K1602028 was digested with XbaI and PstI and ligated into pSB1A2 containing the T7 promoter. Competent E. coli Top 10 were transformed via heat shock. Colonies were screened by colony PCR with VF2 and VR.

Figure 1 Colony PCR of T7-B0034-His-Scaffold. All colonies (1-3) contained the insert of around 1.2 kbp. DNA marker: 2-Log DNA Ladder.