Team:TU Dresden/Notebook/Protocols/Antibiotics


Antibiotics

Name Stock solution Concentration in medium Dilution factor
Ampicillin (Amp) 100 mg/mL 100 μg/mL 1000
Chloramphenicol (Cm) 30 mg/mL 10 μg/mL (lc)
15 μg/mL (lc)
30 μg/mL (hc)
3000
2000
1000
Kanamycin (Kan) 30 mg/mL 15 μg/mL (lc)
50 μg/mL
2000
600
Streptomycin (Str) 50 mg/mL 100 μg/mL 500
Tetracycline (Tet) 10 mg/mL 3 μg/mL (lc, liquid)
5 μg/mL (lc, plate)
3333
2000
X-Gal 20 mg/mL 40 μg/mL 500
lc: low copy plasmids, hc: high copy plasmids.