Team:USTC/Parts
This year, we choose several parts to conduct our projects. The main functions of the function we use are as follow:
- Promoters.
- Proteins to improve the bactial permeability.
- Porins.
- Proteins to improve the mobile of bacteria.
- Sense system.
| Code Name | Part Name | Function |
|---|---|---|
| BBa_K1593207 | micF | Promoter sensing sulfoamid |
| BBa_K1593208 | SoxS | Promoter sensing tetracycline |
| BBa_K1593209 | OprF | The major porin of Pseudomonas aeruginosa allowing diffusion of polysaccharides in a range of 2000 to 3000 Da. |
| BBa_K1593666 | SCVE | SARS Coronavirus Envelope Protein, improving the bactial permeability. |
| BBa_K1593998 | cheZ | Chemotactic protein. When it is overexpressed, the mobility of bacteria will be improved. |
| BBa_K1593667 | T7-RBS-SCVE | Circuit containing strong expression of SCVE regulated by T7, which will significantly improve bacterial permeability. |
| BBa_K1593210 | T7-RBS-OprF | Using T7 strong promoter to express OprF, derived from Pseudomonus areuginosa, would strongly improve the bacterial permeability. |
| BBa_K1593997 | R0010-RBS-cheZ | Used to coding a chemotactci protein, cheZ, downstream of lac promotor. |
| BBa_K1593211 | micF-RBS-GFP | A basic part on antibiotic substance sensing-reporting system. |
| BBa_K1593001 | COMPX | Express an outmembrane protein with a point mutation |
A list of composit parts USTC iGEM 2015 have submitted to the Registry.The main functions of these composite parts are as following:
- To improve the permeability of bacteria;
- To modify the chemotaxis of bacteria;
- To modify outmembrane protein;
- To test the response of promtor.
| Code Name | Parts Name | Type | Function |
|---|---|---|---|
| BBa_K1593667 | T7-RBS-SCVE | Translational_Unit | overexpress SCVE to improve the permeability of bacterial |
| BBa_K1593210 | T7-RBS-OprF | Coding | overexpress OprF to improve the permeability of bacterial |
| BBa_K1593997 | R0010-RBS-cheZ | Coding | overexpress cheZ under the control of lac to improve the chemotaxis of bacterial |
| BBa_K1593211 | micF-RBS-GFP | Translational_Unit | test the response of micF to antibiotics using the GFP report system |
| BBa_K1593888 | tRNA&tRNA Synthetase | Translational_Unit | express a rare amino acid,pAzF |
| BBa_K1593002 | tRNA complex with Compx | Device | orthogonal system to express Compx |
| BBa_K1593000 | lac-compx with RFP | Device | overexpress compx and detect its quantity by GFP |
<groupparts>iGEM015 USTC</groupparts>