Team:UiOslo Norway/Experiments/Ligation
Ligation with T4 DNA Polymerase (NEB):
Back to Protocols2 μl 10X T4 DNA Ligase Buffer 0.020 pmol Vector DNA 0.060 pmol Insert 1 μl T4 DNA Ligase MilliQ Water up to 20 μl
Total Reaction of 20 μl
Incubate at 16 °C overnight
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