Team:York/Protocols/PCR
Colony PCR Protocols
For PCR reactions up to 50 μL add the following to each PCR Tube:
Substance | GoTaq | Q5 | Phusion |
Forward Primer | 2.5 | 2.5 | 2.5 |
Reverse Primer | 2.5 | 2.5 | 2.5 |
Polmerase | .2 | 25 μL mastermix | .5 |
dNTPs | .5 | - | 1 |
dH2O | 14.3 | 18.0 | 33.5 |
Buffer | 5 | - | 10 |
DNA | touch of colony | touch of colony | touch of colony |