Team:York/Protocols/PhosphateAssay Glassmilk

Glassmilk recipe

  1. Stir 400g silica 325 in 800 ml of dH2O
  2. Leave to settle for 90 minutes
  3. Remove supernatant and centrifuge 6000 rpm for 10 minutes
  4. Resuspend pellet in 250 ml dH2O
  5. Add conc. Nitric Acid to 50%
  6. Stir and heat to a boil gently
  7. Stir to RT gently
  8. Centrifuge @ 6000 rpm for 10 minutes (make sure you use either glass or polypropylene tubes, polystyrene tubes can’t withstand over 3000g)
  9. Resuspend in 250 ml dH2O
  10. Repeat spinning and washing until pH is neutral
  11. Resuspend pellet to make 50% by volume slurry

Aliquot and store indefinitely at RT