Template:IONIS Paris/Notebook/27-08-15
Digestion
| Tube | VVD - YC155 | VVD - YN155 |
|---|---|---|
| Buffer 2.1 | 2 µL | 2 µL |
| DNA | 12 µL | 12 µL |
| Enzyme EcoRI | 0,5 µL | - |
| Enzyme PstI | - | 0,5 µL |
| Enzyme XbaI | - | 0,5 µL |
| Enzyme SpeI | 0,5 µL | - |
37°C, 1h heat kill: 80°C, 20 min
Ligation of VVD-YC155 and VVD-YN155 into pSB1C3
| Tube | pSB1C3 |
|---|---|
| Water | 3 µL |
| T4 ligase Buffer | 1 µL |
| VVD-YC155 | 1,3 µL |
| VVD-YN155 | 1,7 µL |
| Plasmid | 2,5 µL |
| T4 ligase | 0,5 µL |
Room temperature, 30min heat kill: 80°C, 20 min
Transformation of bacteria with ligation products
27 August 15