Template:IONIS Paris/Notebook/28-08-15

Results

Result of transformation (26/08):

1 colony onto a gel artefact (no antibiotic) of the control plate;
Few colonies (3 to 20) for others plates excepted H/E plates

• Stored into a fridge for WE, H/E plates stayed at room temperature

Miniprep (kit QIAgen)

Liquid culture of bacteria transformed with BBa_K1159001 plasmid (from iGEM) prepared into 2 columns; final elution 2x15µl/column

PCR of Gblocks (VVD YC, VVD YN, GFP, H/E, CCDB and HOKD)

Mix PCR preparation:

	Gblock
MQ Water	39 µL
RB Buffer	5 µL
Mg2+	1,5 µL
dNTP 10 µM	1 µL
Primer Fwd 25 µM	1µL pSB1C3 Fwd
Primer Rev 25 µM	1µL pSB1C3 Rev
DNA	1µL
Taq Pol enzyme	0,5 µL

Run of the IGEM TAQ 2 program

28 August 15