Template:SJTU-BioX-Shanghai/Notebook/Construction/w6
Construction
Aug. 10
- Prepare 34mg/ml chloramphenicol.
- Propagating cell culture of pCPCG2-HR*-pBluescript and cell conservation.
Aug. 11
- Plasmid extraction.
Aug. 12
- Transformation of BBa_J04450.
Aug. 13
- Inoculation: 10 samples.
Aug. 14
- Plasmid extraction.
- Linearize plasmid by restriction enzyme reaction. Extract the linearized plasmid.
Aug. 16
- Inoculation of DH5α for component cell making.