Template:SJTU-BioX-Shanghai/Notebook/Construction/w6

SJTUB construction logo.jpg Construction

Aug. 10

  1. Prepare 34mg/ml chloramphenicol.
  2. Propagating cell culture of pCPCG2-HR*-pBluescript and cell conservation.

Aug. 11

  1. Plasmid extraction.

Aug. 12

  1. Transformation of BBa_J04450.

Aug. 13

  1. Inoculation: 10 samples.

Aug. 14

  1. Plasmid extraction.
  2. Linearize plasmid by restriction enzyme reaction. Extract the linearized plasmid.

Aug. 16

  1. Inoculation of DH5α for component cell making.