Template:Team:Groningen/CONTENT/EXPERIMENTS/tasA Heat Shock Transformation (t42)
00:00, 8 July 2015 - 00:00, 8 July 2015
Afterwards a heat shock transformation was performed with 100 µL NEB competent cells + ligation product.
90% plate (90µL)
10% plate (10µL)
The plates were incubated overnight at 37 °C.
00:00, 9 July 2015 - 00:00, 9 July 2015
The transformation was checked. On both 10% and 90% plates a large amount of colonies had grown, so the transformation was successful. 4 colonies from the 10% plate were grown overnight in 3 mL LB+cm (6 µL 1/500).