The following constructs were digested.
Template:Team:Groningen/CONTENT/LOGBOOK/GFP Digestion zp103
GFP Digestion (zp103)
Digestion is performed to prepare DNA for ligation. Different constructs were digested to put Psalt in front of different genes, in this case GFP to test the salt inducible promotor.
To create a salt inducible promotor.
Digestion was done.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 August 2015 - 00:00, 26 August 2015
Constructs
Restriction enzymes
Psalt in pSB1C3
SpeI and PstI
GFP+RBS in pSB1C3
XbaI and PstI
Digestion samples.