Template:Team:Groningen/CONTENT/LOGBOOK/GFP Ligation zp40

GFP Ligation (zp40)

Ligation was done to combine GFP with an RBS. This was done to create a construct to test the salt inducible promotor.

To create a salt inducible promotor.

Ligation was successful.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>

00:00, 17 August 2015 - 00:00, 17 August 2015

The following vectors and inserts were ligated.

#

Vector used

Insert

1

RBS in pSB1C3

GFP

Ligated constructs

Marieke Mulder