Template:Team:Groningen/CONTENT/LOGBOOK/Ligation a20
Ligation_a20
Ligating restricted abrB knock out DNA with PsB1A3 backbone.
To knock out abrB in Bacillus subtilis strains to obtain a more rigid biofilm. AbrB suppresses biofilm formation. Knocking out AbrB should result in a more rigid biofilm.
No problems were encounterd during ligation process.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 29 Augustus 2015 - 00:00, 29 Augustus 2015
Ligated PsB1A3 with purified DNA from Jan willem and BD1807.