Template:Team:Groningen/CONTENT/LOGBOOK/Ligation th4
Ligation (th4)
Ligation of thrC locus from pdg1664 with K823023
Extra integration of DNA in thrC locus
The ligation was successful
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
The thrC locus 1 from pdg1664 was ligated with K823023 without AmyE locus 1. The ligation mixture was incubated for 1 hour at room temperature.