The following constructs were digested.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Digestion zp30
Psalt Digestion (zp30)
Digestion is performed to prepare DNA for ligation. Psalt and pSB1C3 are digested to ligate the salt inducible promotor into a backbone.
To create a salt inducible promotor.
Digestion was done.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 17 August 2015 - 00:00, 17 August 2015
Constructs
Restriction enzymes
tasA in pSB1C3
SpeI and PstI
Psalt PCR product
XbaI and PstI
bslA in pSB1C3
SpeI and PstI
pSB1C3
XbaI and PstI
Digestion samples.