The following constructs were digested. After digestion DNA was purified using gel purification on a 1% agarose gel, run at 100V and cut out. Afterwards the samples were purified using a PCR purification kit.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt Digestion zp62
Psalt Digestion (zp62)
Digestion is performed to prepare DNA for ligation. Different constructs were digested to put Psalt with different genes in BBa_K823023
To create a salt inducible promotor.
Digestion was done.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 August 2015 - 00:00, 26 August 2015
Constructs
Restriction enzymes
Psalt in pSB1C3
SpeI and PstI
RBS + GFP in pSB1C3
XbaI and PstI
Psalt + tasA in pSB1C3
EcoRI and PstI
Psalt + bslA in pSB1C3
EcoRI and PstI
BBa_K823023
EcoRI and PstI
Digestion samples.