White colonies from the plate with transformed E. coli were checked for the right insert with the primers seen in table 1. After PCR, sample was loaded on 1% agarose gel with SERVA DNA Stain G 1:50000. Gel results show the correct insert.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt GFP Colony PCR zp78
Psalt GFP Colony PCR (zp78)
A colony PCR was performed to check the insert of a transformed E. coli.
To create a salt inducible promotor.
Gel results show that GFP is integrated into the BBa_K823023 backbone.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/ColonyPCR">Colony PCR</a>
00:00, 29 August 2015 - 00:00, 29 August 2015
Primer
Sequence
VR
attaccgcctttgagtgagc
VF2
tgccacctgacgtctaagaa
Primer sequences used in colony PCR.