Template:Team:Groningen/CONTENT/LOGBOOK/Psalt GFP Ligation zp76

Psalt GFP Ligation (zp76)

Ligation was done to combine the salt inducible promotor (proH) + GFP in a B. subtilis backbone.

To create a salt inducible promotor.

Ligation successful.

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/Ligation">Ligation</a>

00:00, 28 August 2015 - 00:00, 28 August 2015

The following vectors and inserts were ligated.

#

Vector used

Insert

1

BBa_K823023

Psalt + RBS + GFPiv>

Ligated constructs

Marieke Mulder