This was done by growing B. subtilis ComI in LB + chloramphenicol for four hours. Then different salt concentrations were added (0.1M, 0.5M and 1M). This was incubated for 1 hour at 37°C. Afterwards cells were fixated in 1,25% agarose in PBS. Fluorescent microscopy did not show any differences between salt concentrations.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt GFP Validation pz81
Psalt GFP Validation (pz81)
The transformed B. subtilis was tested to check if the salt inducible promotor works by adding different salt concentrations.
To create a salt inducible promotor.
No fluorescent protein visible.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/None">None</a>
00:00, 1 September 2015 - 00:00, 1 September 2015