New primers were designed and ordered.
Template:Team:Groningen/CONTENT/LOGBOOK/Psalt New Primers zp28
Psalt new primer (zp28)
A new reverse primer was designed because the old one had the suffix the wrong way around.
To create a salt inducible promotor.
Ordered successfully.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/None">None</a>
00:00, 24 July 2015 - 00:00, 24 July 2015
Primer
Sequence
salt primer 2 for
CCAGTGAATTCGCGGCCGCTTCTAGAGGTCTGAATTTAAAGAGATTGGTTTTAAACC
salt primer 2 rev
GGGATCCTGCAGCGGCCGCTACTAGTAGATCCTGCTCCTATAAAGGCTACTTTCTTT
Primer sequences