SlrR was ordered from IDT with prefix and suffix. PCR was performed with the prefix and suffix primers.
Prefix primer:
CAGGCAGTTGAATTCGCGGCCGCTTCTAGA
Suffix primer:
CTTGAGCTCCTGCAGCGGCCGCTACTAGTA
The followign thermocycle was observed.
Template:Team:Groningen/CONTENT/LOGBOOK/SlrR PCR sl1
SlrR PCR sl1
PCR to obtain DNA of slrR.
To derepress biofilm formation.
The right bands were observed.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/No_protocol">PCR</a>
00:00, 28 July 2015 - 00:00, 28 July 2015
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Step
Temperature
Time
1
98 °C
5 min
Annealing
55 °C
30 sec
Extension
72 °C
90 sec
3
75 °C
5 min
Thermocycle.
step 2 was repeated 35 times. PCR product was the right fragment and was purified with a PCR purification kit.