Template:Team:Groningen/CONTENT/LOGBOOK/notebookwiebrand

sinR_Transformation_JH642_si1
overexpression of SinR
transformation of JH642 with SinR
failed

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/No_protocol">none</a>

15:48, 24 August 2015 - 24:00, 24 August 2015
MC was placed in the 37°C incubator. The OD was measured at 20:30 but not grown far enough.1 µL of SinR DNA was added to 200 µL of MC with JH642. The culture was plated at 24:00.
Hanneke Siebe
18:00, 25 August 2015 - 24:00, 25 August 2015
No colonies were seen so the transformation failed
Hanneke Siebe