Template:Team:Groningen/CONTENT/LOGBOOK/tasA PCR purification t52
tasA PCR purification (t52)
A PCR purification was performed to get rid of the enzymes used in the PCR process.
Our goal is to overexpress TasA by adding a second copy of the tasA gene under the control of a salt inducible promoter. This overexpression will lead to a stronger biofilm when the biofilm is exposed to salt water.
The sample was purified.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCRpurification">PCR purification</a>
00:00, 23 July 2015 - 00:00, 23 July 2015
The sample was purified using the PCR purification kit. For this 20 µL elution buffer was used.