Template:Team:Groningen/CONTENT/PROTOCOLS/Restriction

Restriction

Component

Plasmid DNA

Unpurified Product

Genomic DNA

Water, nuclease free*

up to 20 μL

up to 20 μL

up to 50 μL

10x NEB 2.1 buffer

2 μL

2 μL**

5 μL

DNA

up to 1 μg

up to 0.2 μg

up to 5 μg

NEB Enzyme

0.5 μL

0.5 μL

0.5 μL

Total Volume

20 μL

20 μL

20 μL

Restriction.