Template:Team:Groningen/CONTENT/Protocols and Protocols/Transformation

Transformation

Protocol for the transformation of Bacillus subtilis.

ML-I

Plating Bacillus subtilis

Day 1: streak out Bacillus subtilis strain on a plate and incubate plate overnight at 37 ℃.

Inoculating Bacillus subtilis

Day 2: pick a colony from the plate and add it to 2 mL of MC completed.

Place the mixture in 37 ℃ incubator for 5 hours.

Afterwards, mix 400 µL of culture with 1 µL DNA in a 15 mL tube. Close the tube loosely.

Let these bacteria grow for 2 hours at 37℃.

Plating transformed Bacillus subtilis

Lastly, plate the complete content of culture on selective antibiotic plates and incubate at 37℃ overnight .