LOCUS       BBa_K1621002            2460 bp    DNA     circular UNA 07-SEP-2015
DEFINITION  A new nucleotide sequence entered manually.
ACCESSION   urn.local...19h-5072g35
VERSION     urn.local...19h-5072g35
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    230..250
     gene            253..642
     misc_feature    643..662
     stem_loop       663..734
                     /note="Geneious type: misc_feature"
     misc_feature    complement(796..815)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(902..1516)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       1683..1788
                     /note="Geneious type: misc_feature"
     CDS             complement(1801..2460)
                     /gene="Chloramphenicol resistance"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga tgaccactgg tcggccttct catgaagcac cgaatatgac ccaaactggc
      301 accacagaca gcccgaccgc aattagcttg accacacccg accacacgcc accgatgccg
      361 agcattggtt tagaggaaga agaggaagag gaaggcgctg gcgatggtga acatctggaa
      421 gggggagacg gtacacgcga caccctgccg cagagcccgg gccctgcgtt tccgctggcc
      481 gaagacgtag agaaagataa acctaaccgc ccggttgttc cgtctcccga tcctaataat
      541 tcccctgctc gtccagaaac ctcgcgcccc aagaccccgc caacaattat cggtccgctg
      601 gccacgcgtc caaccacccg tttaacaagc aaaggtcgcc caactagtag cggccgctgc
      661 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
      721 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      901 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg
     1681 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1741 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1801 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1861 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1921 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1981 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     2041 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     2101 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     2161 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     2221 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     2281 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     2341 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     2401 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
//
