LOCUS       BBa_I20270_             2989 bp    DNA     circular UNA 18-SEP-2015
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...z-565zr4m (modified)
VERSION     urn.local...z-565zr4m
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     112..131
     misc_feature    230..>249
     gene            252..1170
     misc_feature    <1173..1191
     stem_loop       1192..1263
                     /note="Geneious type: misc_feature"
     misc_feature    complement(1325..1344)
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1431..2045)
                     /note="Geneious type: misc"
                     /gene="rep (pMB1)"
                     /note="Geneious type: misc_feature"
     stem_loop       2212..2317
                     /note="Geneious type: misc_feature"
     misc_feature    complement(2330..2989)
                     /note="Geneious type: CDS; cds"
                     /gene="Chloramphenicol resistance"
                     /note="Geneious type: CDS"
ORIGIN      
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gttgatggct agctcagtcc taggtacaat gctagctact agagtcacac
      301 aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      361 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      421 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      481 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      541 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      601 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      661 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      721 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      781 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      841 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      901 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      961 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1021 atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1081 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1141 caccttcggg tgggcctttc tgcgtttata tactagtagc ggccgctgca gtccggcaaa
     1201 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
     1261 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1441 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     2221 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     2281 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     2341 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2401 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2461 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2521 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     2581 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2641 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     2701 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     2761 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     2821 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2881 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2941 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccat
//
