LOCUS       pET_1704                6066 bp    DNA     circular UNA 18-SEP-2015
DEFINITION  HIV antigen with Spy- and 6xHis-tag.
ACCESSION   urn.local...f-563ws0h (modified)
VERSION     urn.local...f-563ws0h
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    387..405
                     /note="Geneious type: promoter prokaryotic"
                     /note="Geneious type: misc_feature"
     misc_feature    406..430
     unsure          450..466
                     /note="Geneious type: misc_feature"
     misc_feature    475..540
     unsure          571..588
     gene            598..1134
     unsure          1144..1182
     terminator      1265..1311
                     /note="Geneious type: misc_feature"
     rep_origin      1348..1803
     CDS             1935..2792
                     /note="Geneious type: cds"
     rep_origin      2950..3569
     misc_feature    3654..3673
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(4987..6066)
                     /note="Geneious type: cds"
ORIGIN      
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat acatatgaaa
      481 tacctgctgc cgaccgctgc tgctggtctg ctgctcctcg ctgcccagcc ggcgatggcc
      541 atggatatcg gaattaattc gggtaccatg catcaccatc accatcacgg cggatccggg
      601 attagctatg gccgcaagaa acgtcgccaa cgtcgtcgtg cgcatcagaa catggaacca
      661 gtagacccgc gtctggagcc gtggaaacat ccgggctctc agccgaaaac cgcagcgtat
      721 ccgcaaggat ggaaaggaag cccggcgatt ttccagtcta gcatgaccaa aatcctggag
      781 ccattccgga aacaaaaccc ggacattgtc atttatcaat acatggatga tttgtacgtg
      841 ggtgcggcag cgaaggagcc gttccgtgat tacgtagatc gtttttataa aacccttcgg
      901 gctgaacaag caagccagga agttaagaac tggatgacgg cggcctatca ggctcgcatt
      961 ttggcggtag aacgttacct gaaagaccaa cagctgctgg gcatttgggg ctgttcagga
     1021 aagctgatct gcacgactgc ggtgccgtgg tgtacgcgtc cgaacaacaa cacccgcaaa
     1081 cgtatccgta ttcaacgggg tcctggtcgc gcgttcgtaa ccattggaaa gatcaagctt
     1141 ggcgctcata ttgtcatggt tgatgcttac aagccaacta agtagcttaa gttgagatga
     1201 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1261 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     1321 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     1801 tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1861 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1921 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1981 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2041 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2101 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2161 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2221 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2281 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2341 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2401 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2461 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     2521 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2581 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2641 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2701 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2761 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2821 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2881 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2941 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3001 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3061 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     3121 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3181 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3241 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3301 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3361 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3421 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3481 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3541 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3601 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3661 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3721 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3781 cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3841 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     3901 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     3961 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4021 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     4081 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     4141 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     4201 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     4261 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     4321 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     4381 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     4441 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     4501 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     4561 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     4621 agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg
     4681 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     4741 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     4801 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
     4861 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     4921 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     4981 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     5041 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
     5101 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
     5161 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat
     5221 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg
     5281 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag
     5341 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat
     5401 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt
     5461 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc
     5521 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg
     5581 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac
     5641 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat
     5701 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc
     5761 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat
     5821 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag
     5881 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
     5941 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac
     6001 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg
     6061 tttcac
//
