View source for Team:CityU HK/Notebook

{{:Team:CityU_HK/Template/Meun}}
<html>
	<head>
		<title>Description - iGEM2015 wiki</title>


<meta http-equiv="Content-Type" content="text/html; charset=utf-8"/>
<meta name="viewport" content="width=device-width, initial-scale=1.0"/>

		
<link rel="stylesheet" type="text/css" href="http://cdn2.editmysite.com/css/sites.css?buildTime=1234" />
<link rel="stylesheet" type="text/css" href="http://cdn1.editmysite.com/editor/libraries/fancybox/fancybox.css?1234" />
<link rel="stylesheet" type="text/css" href="http://cdn2.editmysite.com/css/social-icons.css?buildtime=1234" media="screen,projection" />
<link rel="stylesheet" type="text/css" href="files/main_style.css?1440597364" title="wsite-theme-css" />
<link href='http://fonts.googleapis.com/css?family=Lato:400,300,300italic,700,400italic,700italic&subset=latin,latin-ext' rel='stylesheet' type='text/css' />
<link href='http://fonts.googleapis.com/css?family=Fjalla+One&subset=latin,latin-ext' rel='stylesheet' type='text/css' />

<style type='text/css'>

/************* sidebar style **************/
@import url(//netdna.bootstrapcdn.com/font-awesome/4.0.3/css/font-awesome.css);
}
@import url(http://fonts.googleapis.com/css?family=Titillium+Web:300);
.fa-2x {
font-size: 2em;
}
.sidebar-fa {
position: relative;
display: table-cell;
width: 60px;
height: 36px;
text-align: center;
vertical-align: middle;
font-size:20px;
color:#ffffff;
}

.main-menu:hover,nav.main-menu.expanded {
width:250px;
overflow:visible;
}

.main-menu {
background:#ffb90f;
border-right:1px solid #e5e5e5;
position:fixed;
top:200px;
bottom:0;
height:25%;
left:0;
width:60px;
overflow:hidden;
-webkit-transition:width .05s linear;
transition:width .05s linear;
-webkit-transform:translateZ(0) scale(1,1);
z-index:1000;
}

.main-menu>ul {
margin:7px 0;
}

.main-menu li {
position:relative;
display:block;
width:250px;
}

.main-menu li>a {
position:relative;
display:table;
border-collapse:collapse;
border-spacing:0;
color:#999;
 font-family: arial;
font-size: 14px;
text-decoration:none;
-webkit-transform:translateZ(0) scale(1,1);
-webkit-transition:all .1s linear;
transition:all .1s linear;
  
}

.main-menu .nav-icon {
position:relative;
display:table-cell;
width:60px;
height:36px;
text-align:center;
vertical-align:middle;
font-size:18px;
}

.main-menu .nav-text {
position:relative;
display:table-cell;
vertical-align:middle;
width:190px;
font-family: 'Titillium Web', sans-serif;
}

.main-menu>ul.logout {
position:absolute;
left:0;
bottom:0;
}

.no-touch .scrollable.hover {
overflow-y:hidden;
}

.no-touch .scrollable.hover:hover {
overflow-y:auto;
overflow:visible;
}

a:hover,a:focus {
text-decoration:none;
}

nav {
-webkit-user-select:none;
-moz-user-select:none;
-ms-user-select:none;
-o-user-select:none;
user-select:none;
}

nav ul,nav li {
outline:0;
margin:0;
padding:0;
}
.main-menu li:hover>a,nav.main-menu li.active>a,.dropdown-menu>li>a:hover,.dropdown-menu>li>a:focus,.dropdown-menu>.active>a,.dropdown-menu>.active>a:hover,.dropdown-menu>.active>a:focus,.no-touch .dashboard-page nav.dashboard-menu ul li:hover a,.dashboard-page nav.dashboard-menu ul li.active a {
color:#fff;
background-color:#5fa2db;
}
.area {
float: left;
background: #e2e2e2;
width: 100%;
height: 100%;
}
.nav-text {
font-weight:bold;
font-size:1.2em;
color:#ffffff;
border-top-style: solid;
border-top-width: 1px;
border-top-color: #f9d682;
}
#first-item {
border:none;
}

@font-face {
  font-family: 'Titillium Web';
  font-style: normal;
  font-weight: 300;
  src: local('Titillium WebLight'), local('TitilliumWeb-Light'), url(http://themes.googleusercontent.com/static/fonts/titilliumweb/v2/anMUvcNT0H1YN4FII8wpr24bNCNEoFTpS2BTjF6FB5E.woff) format('woff');
}
</style>

/* ****** Back to top button ******* */
<style>

#back-top {
	position: fixed;
	bottom: 30px;
        right:30px;
        z-index:50;
}
#back-top a {
	width: 50px;
	display: block;
	text-align: center;
	font: 11px/100% Arial, Helvetica, sans-serif;
	text-transform: uppercase;
	text-decoration: none;
	color: #bbb;
	/* background color transition */
	-webkit-transition: 1s;
	-moz-transition: 1s;
	transition: 1s;
        border-radius: 2px;
}
#back-top a:hover {
	color: #000;
}
/* arrow icon (span tag) */
#back-top span {
	width: 50px;
	height: 50px;
	display: block;
	background: #ddd url(https://static.igem.org/mediawiki/2014/5/57/Backtotop_BU14.png) no-repeat center center;
	/* background color transition */
	-webkit-transition: 1s;
	-moz-transition: 1s;
	transition: 1s;
border-radius: 50%;
}
#back-top a:hover span {
	background-color: #ffb90f;
}
</style>
<style>
.wsite-background {background-image: url('https://static.igem.org/mediawiki/2015/f/f9/2015CityU_HK_notebook_banner.png') !important;background-repeat: no-repeat !important;background-position: 50% 82.7300033569336% !important;background-size: 100% !important;background-color: transparent !important;}
body.wsite-background {background-attachment: fixed !important;}.wsite-background.wsite-custom-background{ background-size: cover !important}
.main-wrap{margin-left:auto;margin-right:auto;width:80%;}
</style>
</head>

	

<body class="short-header-page  wsite-page-description"><input type="checkbox" id="navTrigger" />

  <div class="wrapper">
 
	        
		<div class="banner-wrap wsite-background wsite-custom-background">
			<div class="container">
				<div class="banner">
					<h2 style="color:"#F5F5F5"><span class="wsite-text wsite-headline">
	                                Notebook
                                        </span></h2>
				</div>
			</div>
		</div>
	

<!----------- sidebar html ------------>
<div class="area"></div><nav class="main-menu">
            <ul>
                <li>
                    <a href="#laczy">
                      <i class="sidebar-fa"></i>
                        <span class="nav-text" id="first-item">
                            LacZY plasmid
                        </span>
                    </a>        
                </li>

                <li class="has-subnav">
                    <a href="#lysis-lambda">
                      <i class="sidebar-fa fa fa-th-list fa-2x"></i>
                        <span class="nav-text">
                            Lysis plasmid (λ phage)
                        </span>
                    </a>                 
                </li>

                <li class="has-subnav">
                    <a href="#lysis-21">
                      <i class="sidebar-fa"></i>
                        <span class="nav-text">
                            Lysis plasmid (Phage 21)
                        </span>
                    </a>     
                </li>

                <li class="has-subnav">
                    <a href="#interlab">
                      <i class="sidebar-fa"></i>
                        <span class="nav-text">
                            Interlab study
                        </span>
                    </a>
                </li>
            </ul>

        </nav>   <!---------end of sidebar --------->


<!-- main content starts here -->
		<div id="main-wrap">
			<div class="container">
				<div id='wsite-content' class='wsite-elements wsite-not-footer'>


<div class="wsite-spacer" style="height:50px;"></div>

         <td class="wsite-multicol-col" style="width:9.5959595959596%; padding:0 15px;">
                   <center><h2>Lab Log</h2></center>

<div class="wsite-spacer" style="height:50px;"></div>

<divid="laczy" ><div style="height: 20px; overflow: hidden; width: 100%;"></div>
<hr class="styled-hr" style="width:100%;"></hr>
<div style="height: 20px; overflow: hidden; width: 100%;"></div></div>

<div class="paragraph" style="text-align:left;"><font size="5">lacZY plasmid</font></div></div>
<hr class="styled-hr" style="width:100%;"></hr>

<div><div style="height: 20px; overflow: hidden; width: 100%;"></div>

<p>Keys to the table: <br>

[?/?]: Names of restriction enzyme used for digestion:; E, EcoRI; X, XbaI; S, SpeI; P, PstI <br>

- A ribosome binding site (RBS) is linked upstream to each gene <br>

- The host cells used for transformation were competent JM109 <i>E. coli</i> cells </p>

<style>
table {
    width:100%;
}

th, td {
    padding: 5px;
    text-align: left;
}
table#t01 tr:nth-child(even) {
    background-color: #eee;
}
table#t01 tr:nth-child(odd) {
   background-color:#fff;
}
table#t01 th	{
    background-color: black;
    color: white;
}
</style>

<br>
<p>PCR amplification of the lacZ and lacY genes.</p>
<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 1 : May 18 – 22</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td>Group 1</td>		
    <td>Group 2</td>
    <td>Group 3</td>
    <td>Group 4</td>
  </tr>
  <tr>
    <td>Monday 18</td>
    <td colspan="4"><center>==introduction==</center></td>		
  </tr>
  <tr>
    <td bgcolor="#FFB2D1">Tuesday 19</td>
    <td colspan="4" bgcolor="#FFB2D1"></td>
  </tr>
  <tr>
    <td>Wednesday 20</td>
    <td><li>PCR amplification of the BBa_S04055 <i>lacZ</i> gene</li></td>		
    <td><li>PCR amplification of the BBa_S04055 <i>lacZ</i> gene</li></td>
    <td><li>PCR amplification of the BBa_S04055 <i>lacY</i> gene</li></td>
    <td><li>PCR amplification of the BBa_S04055 <i>lacY</i> gene</li></td>
  </tr>
  <tr>
    <td>Thursday 21</td>
    <td><li>Gel electrophoresis of the amplicon</li> <li><i>lacZ</i> PCR product purification </li> <li>Restriction digestion with [E/P] of the vector pSB1C3 & <i>lacZ</i></li></td>		
    <td><li>Gel electrophoresis of the amplicon</li> <li><i>lacZ</i> PCR product purification </li> <li>Restriction digestion with [E/P] of the vector pSB1C3 & <i>lacZ</i></li></td>	
    <td><li>Gel electrophoresis of the amplicon</li> <li><i>lacY</i> PCR product purification </li> <li>Restriction digestion with [E/P] of the vector pSB1C3 & <i>lacY</i></li></td>	
    <td><li>Gel electrophoresis of the amplicon</li> <li><i>lacY</i> PCR product purification </li> <li>Restriction digestion with [E/P] of the vector pSB1C3 & <i>lacY</i></li></td>
  </tr>
  <tr>
    <td>Friday 22</td>
    <td><li>Gel purification of vector pSB1C3 [E/P] digest</li> <li>Ligation of the [E/P] digested <i>lacZ</i> insert into the [E/P] digested vector pSB1C3</li></td>		
    <td><li>Gel purification of vector pSB1C3 [E/P] digest</li> <li>Ligation of the [E/P] digested <i>lacZ</i> insert into the [E/P] digested vector pSB1C3</li></td>
    <td><li>Gel purification of vector pSB1C3 [E/P] digest</li> <li>Ligation of the [E/P] digested <i>lacY</i> insert into the [E/P] digested vector pSB1C3</li></td>
    <td><li>Gel purification of vector pSB1C3 [E/P] digest</li> <li>Ligation of the [E/P] digested <i>lacY</i> insert into the [E/P] digested vector pSB1C3</li></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 2 : May 25 – 29</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td>Group 1</td>		
    <td>Group 2</td>
    <td>Group 3</td>
    <td>Group 4</td>
  </tr>
  <tr>
    <td bgcolor="#FFB2D1">Monday 25</td>
    <td colspan="4" bgcolor="#FFB2D1"></td>		
  </tr>
  <tr>
    <td>Tuesday 26</td>
    <td><li>Transformation of May 22 ligation product into competent <i>E. coli</i> cells</li></td>
    <td><li>Transformation of May 22 ligation product into competent <i>E. coli</i> cells</li></td>
    <td><li>Transformation of May 22 ligation product into competent <i>E. coli</i> cells</li></td>
    <td><li>Transformation of May 22 ligation product into competent <i>E. coli</i> cells</li></td>
  </tr>
  <tr>
    <td>Wednesday 27</td>
    <td><li>Colony PCR of May 26 transformed cells</li></td>		
    <td><li>Colony PCR of May 26 transformed cells</li></td>
    <td><li>Colony PCR of May 26 transformed cells</li></td>
    <td><li>Colony PCR of May 26 transformed cells</li></td>
  </tr>
  <tr>
    <td>Thursday 28</td>
    <td><li>Extraction of the plasmid pSB1C3-BBa_J23100 </li> <li>Restriction digestion with [S/P] of the vector pSB1C3-BBa_J23100</li></td>		
    <td><li>Extraction of the plasmid pSB1C3-BBa_J23100 </li> <li>Restriction digestion with [S/P] of the vector pSB1C3-BBa_J23100</li></td>	
    <td><li>Extraction of the plasmid pSB1C3-BBa_J23100 </li> <li>Restriction digestion with [S/P] of the vector pSB1C3-BBa_J23100</li></td>	
    <td><li>Extraction of the plasmid pSB1C3-BBa_J23100 </li> <li>Restriction digestion with [S/P] of the vector pSB1C3-BBa_J23100</li></td>
  </tr>
  <tr>
    <td>Friday 29</td>
    <td><li>Gel purification of May 28 digest</li></td>		
    <td><li>Gel purification of May 28 digest</li></td>
    <td><li>Gel purification of May 28 digest</li></td>
    <td><li>Gel purification of May 28 digest</li></td>
  </tr>
</table>

<br><br>
<p>Groups 1 & 3 and Groups 2 & 4 used different cloning strategies to assemble the biobrick J23100_lacZ-lacY.</p>
<br>
<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 3 : June 1 – 5</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td>Group 1</td>		
    <td>Group 3</td>
    <td>Group 2</td>
    <td>Group 4</td>
  </tr>
  <tr>
    <td>Monday 1</td>
    <td><li>Extraction of the plasmid pSB1C3-<i>lacZ</i> (from May 26 transformed cells)</li> <li>Restriction digestion with [X/P] of the insert <i>lacZ</i></li> <li>Gel purification</li></td>
    <td><li>Extraction of the plasmid pSB1C3-<i>lacY</i> (from May 26 transformed cells)</li> <li>Restriction digestion with [X/P] of the insert <i>lacY</i></li> <li>Gel purification</li></td>
    <td><li>Extraction of the plasmid pSB1C3-<i>lacZ</i> from Gp1</li> <li>Restriction digestion with [E/S] of the insert <i>lacZ</i></li> <li>Gel purification</li></td>
    <td><li>Extraction of the plasmid pSB1C3-<i>lacY</i> from Gp1</li> <li>Restriction digestion with [E/S] of the insert <i>lacY</i></li> <li>Gel purification</li></td>		
  </tr>
  <tr>
    <td>Tuesday 2</td>
    <td><li>Redo the work on June 1</li></td>
    <td><li>Redo the work on June 1</li></td>
    <td><li>Redo the work on June 1</li></td>
    <td><li>Redo the work on June 1</li></td>
  </tr>
  <tr>
    <td>Wednesday 3</td>
    <td colspan="2"><li>Ligation of Gp1 [X/P] digested <i>lacZ</i> insert (from June 2) into [S/P] digested vector pSB1C3-BBa_J23100 (from May 29)</li> <li>Transformation</li></td>		
    <td colspan="2"><li>Ligation of Gp2 [E/S] digested <i>lacZ insert</i> (from June 2) into Gp4 [E/X] digested vector pSB1C3-<i>lacY</i>(from June 2)</li> <li>Transformation</li></td>    
  </tr>
  <tr>
    <td>Thursday 4</td>
    <td colspan="2"><li>Colony PCR on June 3 transformed cells</li></td>		
    <td colspan="2"><li>Colony PCR on June 3 transformed cells</li></td>
  </tr>
  <tr>
    <td>Friday 5</td>
    <td colspan="2"><li>Extraction of plasmid pSB1C3-BBa_J23100-<i>lacZ</i> (from June 3 transformed cells)</li> <li>Restriction digestion with [S/P] of the vector pSB1C3-BBa_J23100-<i>lacZ</i></li> <li>Gel purification</li></td>
    <td colspan="2"><li>Extraction of plasmid pSB1C3-<i>lacZ</i>-<i>lacY</i> (from June 3 transformed cells)</li> <li>Restriction digestion with [X/P] of the insert <i>lacZ-lacY</i> <li>Gel purification</li></td>	
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 4 : June 8 – 12</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td>Group 1</td>		
    <td>Group 3</td>
    <td>Group 2</td>
    <td>Group 4</td>
  </tr>
  <tr>
    <td>Monday 8</td>
    <td colspan="2"><li>Ligation of Gp3 [X/P] digested <i>lacY</i> insert (from June 2) into [S/P] digested vector pSB1C3-BBa_J23100-<i>lacZ</i> (from June 5)</li> <li>Transformation</li></td>
    <td colspan="2"><li>Ligation of [X/P] digested <i>lacZ</i>-<i>lacY</i> insert (from June 5) into [S/P] digested vector pSB1C3-BBa_J23100 (from May 29)</li> <li>Transformation</li></td>		
  </tr>
  <tr>
    <td>Tuesday 9</td>
    <td colspan="2"><li>Colony PCR of June 8 transformed cells (failed)</li></td>
    <td colspan="2"><li>Colony PCR of June 8 transformed cells (failed)</li></td>
  </tr>
  <tr>
    <td>Wednesday 10</td>
    <td colspan="2"><li>Extraction of the plasmid pSB1C3-BBa_J23100-<i>lacZ</i>-<i>lacY</i> (from June 8 transformed cells)</li> <li>Restriction analysis with [E/P] for confirmation of the insert size</li></td>		
    <td colspan="2"><li>Extraction of the plasmid pSB1C3-BBa_J23100-<i>lacZ</i>-<i>lacY</i> (from June 8 transformed cells)</li> <li>Restriction analysis with [E/P] for confirmation of the insert size</li></td>    
  </tr>
</table>

<br>
<center><u>Characterization of the lacZY plasmid</u></center>
<br>

<table id="t01" border="1">
  <tr>
    <th colspan="3">Week 8 : July 6 – 10</th>
  </tr>
  
  <tr>
    <td>Date</td>
    <td></td>		
    <td>Remarks</td>    
  </tr>
  <tr>
    <td>Monday 6</td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>Tuesday 7</td>
    <td><li>RNA extraction from the <i>E. coli</i> cells harboring the recombinant plasmid pSB1C3-BBa_J23100- <i>lacZ</i>-<i>lacY</i></li></td>
    <td>Not successful</td>
  </tr>
  <tr>
    <td>Wednesday 8</td>
    <td><li>Redo RNA extraction</li></td>
    <td>Not successful</td>
  </tr>
  <tr>
    <td>Thursday 9</td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>Friday 10</td>
    <td><li>Redo RNA extraction</li></td>
    <td>Not successful</td>
  </tr>

</table>

<table id="t01" border="1">
  <tr>
    <th colspan="3">Week 9 : July 13 – 18</th>
  </tr>
  
  <tr>
    <td>Date</td>
    <td></td>		
    <td>Remarks</td>    
  </tr>
  <tr>
    <td>Monday 13</td>
    <td><li>Redo the RNA extraction</li></td>
    <td>Successful</td>
  </tr>
  <tr>
    <td>Tuesday 14</td>
    <td><li>Extract RNA from control <i>E. coli</i> cells</li></td>
    <td>Successful</td>
  </tr>
  <tr>
    <td>Wednesday 15</td>
    <td><li>Redo RNA extraction from control <i>E. coli</i> cells</li> <li>Perform RT-PCR on purified RNA from recombinant and control <i>E. coli</i></li></td>
    <td>Successful</td>
  </tr>
  <tr>
    <td>Thursday 16</td>
    <td><li>Check the size of the RT-PCR product using gel electrophoresis</li></td>
    <td>Successful</td>
  </tr>
  <tr>
    <td>Friday 17</td>
    <td><li>Prepare Z-buffer</li></td>
    <td></td>
  </tr>
  <tr>
    <td>Saturday 18</td>
    <td><li>Perform qPCR on the control cDNA</li></td>
    <td>Successful</td>

</table>

<table id="t01" border="1">
  <tr>
    <th colspan="3">Week 10 : July 20 – 25</th>
  </tr>
  
  <tr>
    <td>Date</td>
    <td></td>		
    <td>Remarks</td>    
  </tr>
  <tr>
    <td>Monday 20</td>
    <td><li>Perform q-PCR on recombinant cDNA</li></td>
    <td>Successful</td>
  </tr>
  <tr>
    <td>Tuesday 21</td>
    <td rowspan="4"><li>Perform ONPG assay -characterization on the expression level of LacZ protein</li></td>
    <td rowspan="4"></td>
  </tr>
  <tr>
    <td>Wednesday 22</td>
    
  </tr>
  <tr>
    <td>Thursday 23</td>
    
  </tr>
  <tr>
    <td>Friday 24</td>
    
  </tr>
</table>  <!-- end of lacZY -->




<!---------lysis 21 --------->
<div  id="lysis-21"><div style="height: 20px; overflow: hidden; width: 100%;"></div>
<hr class="styled-hr" style="width:100%;"></hr>
<div style="height: 20px; overflow: hidden; width: 100%;"></div></div>  

<div class="paragraph" style="text-align:left;"><font size="5">Lysis plasmid (phage 21)</font></div></div>
<hr class="styled-hr" style="width:100%;"></hr>

<div><div style="height: 20px; overflow: hidden; width: 100%;"></div>

<p>Keys to the table: <br>

<b>ori</b>: original gene sequence, without codon optimization <br>

<b>co</b>: codon optimized <br>

<b>21Lysis(o_o_o):</b> <i>S21WT(ori)-Rλ(ori)-Rzλ(ori)</i><br>

<b>21Lysis(c_c_c):</b> <i>S21WT(co)-Rλ(co)-Rzλ(co)</i><br>

<b>21Lysis_Sm(o_c_c):</b> <i>S21mut(ori)-Rλ(co)-Rzλ(co)</i><br>

<b>21Lysis_Sm(c_c_c):</b> <i>S21mut(co)-Rλ(co)-Rzλ(co)</i><br>

[x/y]: restriction sites digested: E, EcoRI; X, XbaI; S, SpeI; P, PstI <br>

Gene name or plasmid name [x/y]: the gene fragment or plasmid flanked with the restriction sites x and y at the ends <br>

-A ribosome binding site (RBS) is linked upstream of each gene <br>

-Coloring refers to sub-tasks in that particular group <br>

-The cells used for transformation were competent JM109 <i>E. coli</i> cells</p>

<style>
table {
    width:100%;
}

th, td {
    padding: 5px;
    text-align: left;
}
table#t01 tr:nth-child(even) {
    background-color: #eee;
}
table#t01 tr:nth-child(odd) {
   background-color:#fff;
}
table#t01 th	{
    background-color: black;
    color: white;
}
</style>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 4 : June 8 – 12</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 8</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>		
  </tr>
  <tr>
    <td>Tuesday 9</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>		
  </tr>
  
  <tr>
    <td>Wednesday 10</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li>Extraction of the plasmid pGOv4-<i>Rz21(co)</i> by mini-prep</li><li>Restriction digestion with [E/P]</li></td>
  </tr>
  <tr>
    <td>Thursday 11</td>
    <td></td>		
    <td></td>	
    <td></td>	
    <td><li>Gel purification of the June 10 [E/P] digested <i>Rz21(co)</i></li><li>Ligation of the [E/P] digested <i>Rz21(co)</i> insert into [E/P] digested pSB1C3</li><li>Transformation</li></td>
  </tr>
  <tr>
    <td>Friday 12</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li>Colony PCR on <i>Rz21(co)</i> clones showed bands of the expected size</li></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 5 : June 15 – 19</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 15</td>
    <td colspan="4" bgcolor="#FFB2D1"></td>	
  </tr>
  <tr>
    <td>Tuesday 16</td>
    <td><li>Plasmid extraction of the pGOv4-<i>R21(co)</i></li><li>Restriction digestion with [E/P]</li></td>
    <td></td>
    <td></td>
    <td></td>		
  </tr>
  
  <tr>
    <td>Wednesday 17</td>
    <td><li>Gel purification of the [E/P] digested <i>R21(co)</i></li><li>Ligation of the [E/P] digested <i>R21(co)</i> insert into the [E/P] digested pSB1C3</li><li>Transformation</li></td>		
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>Thursday 18</td>
    <td><li>Colony PCR confirmed the presence of the <i>R21(co)</i> insert</li></td>		
    <td></td>	
    <td></td>	
    <td></td>
  </tr>
  <tr>
    <td>Friday 19</td>
    <td></td>		
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 6 : June 22 – 26</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 22</td>
    <td><li>Extraction of June 17 plasmid pSB1C3-<i>R21(co)</i></li></td>
    <td></td>
    <td></td>
    <td></td>		
  </tr>
  <tr>
    <td>Tuesday 23</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li>Extraction of June 11 plasmid pSB1C3-<i>Rz21(co)</i></li><li>Restriction analysis with [E/P]</li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 24</td>
    <td></td>		
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>Thursday 25</td>
    <td></td>		
    <td></td>	
    <td></td>	
    <td></td>
  </tr>
  <tr>
    <td>Friday 26</td>
    <td></td>		
    <td></td>
    <td><li>PCR amplification on phage 21 wild type lysis cassette</li></td>
    <td></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 7 : June 29 – July 3</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 29</td>
    <td><li>Restriction digestion of June 22 <i>R21(co)</i> with [S/P] & Gel purification</li></td>
    <td></td>
    <td></td>
    <td><li>Extraction of June 11 plasmid pSB1C3-<i>Rz21(co)</i></li> <li>Restriction digestion with [X/P]</li></td>		
  </tr>
  <tr>
    <td>Tuesday 30</td>
    <td></td>
    <td></td>
    <td><li>Redo the PCR amplification on phage 21 wild type lysis cassette</li></td>
    <td><li>Redo June 29’s restriction digestion of <i>Rz21(co)</i> with [X/P]</li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 1</td>
    <td colspan="4" bgcolor="#FFB2D1"></td>		
    </tr>
  <tr>
    <td>Thursday 2</td>
    <td></td>		
    <td></td>	
    <td></td>	
    <td><li>Gel purification of [X/P] digested <i>Rz21(co)</i></li> <li>Ligation of the [X/P] digested <i>Rz21(co)</i> insert into Gp1 June 29 [S/P] digested pSB1C3-<i>R21(co)</i> in [S/P]</li></td>
  </tr>
  <tr>
    <td>Friday 3</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li>Transformation of July 2 ligation product pSB1C3-<i>R21(co)-Rz21(co)</i></li></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 8 : July 6 – July 10</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 6</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li>Colony PCR on July 3 <i>R21(co)-Rz21(co)</i> clones. No bands were observed</li></td>		
  </tr>
  <tr>
    <td>Tuesday 7</td>
    <td></td>
    <td></td>
    <td><li>Extraction of June 30 plasmid pSB1C3-<i>S21WT(ori)-R21(ori)-Rz21(ori)</i> (21Lysis(o_o_o))</li> <li>Restriction digestion with [X/P]</li></td>
    <td><li>Redo July 6's colony PCR with other colonies. No bands were observed</li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 8</td>
    <td></td>
    <td></td>
    <td><li>Redo the restriction digestion of 21Lysis(o_o_o) with [X/P]</li></td>
    <td></td>		
    </tr>
  <tr>
    <td>Thursday 9</td>
    <td></td>		
    <td></td>	
    <td><li>Gel purification of July 8 [X/P] digested 21Lysis(o_o_o)</li></td>	
    <td></td>
  </tr>
  <tr>
    <td>Friday 10</td>
    <td></td>		
    <td></td>
    <td><li>Ligation of the [X/P] digested 21Lysis(o_o_o) insert into [X/P] digested pSB1C3 vector</li> <li>Transformation</li></td>
    <td><li>Restriction analysis on July 3 <i>R21(co)-Rz21(co)</i> clones. The analysis confirmed the presence of the <i>R21(co)-Rz21(co)</i> insert</li></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 9 : July 13 – July 18</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 13</td>
    <td></td>
    <td></td>
    <td><li>Colony PCR on July 10 21Lysis(o_o_o) clones. No bands were observed</li></td>
    <td></td>		
  </tr>
  <tr>
    <td>Tuesday 14</td>
    <td></td>
    <td></td>
    <td><li>Restriction analysis on the 21Lysis(o_o_o) clones. The analysis confirmed the presence of the 21Lysis(o_o_o) insert</li></td>
    <td><li>Extraction of the plasmid pSB1C3-<i>R21(co)-Rz21(co)</i></li> <li>Restriction digestion with [X/P]</li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 15</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li>Gel purification of July 14 [X/P] digested <i>R21(co)-Rz21(co)</i></li></td>		
    </tr>
  <tr>
    <td>Thursday 16</td>
    <td></td>		
    <td></td>	
    <td></td>	
    <td></td>
  </tr>
  <tr>
    <td>Friday 17</td>
    <td></td>		
    <td></td>
    <td></td>
    <td></td>
  </tr>
  <tr>
    <td>Saturday 18</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li><font color="#D633FF">Extraction of the plasmids pSB1C3-<i>S21mut(ori)</i> and pSB1C3-<i>S21mut(co)</i></li> <li>Restriction digestion wth [S/P]</font></li></td>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 10 : July 20 – July 25</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 20</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li><font color="#D633FF">Gel purification of July 18 [S/P] digested pSB1C3-<i>S21mut(ori)</i> and pSB1C3-<i>S21mut(co)</i></font></li> <br> <li><font color="#0099FF">Ligation of July 15 [X/P] digested <i>R21(co)-Rz21(co)</i> insert into [S/P] digested pSB1C3-<i>S21mut(ori)</i></font></li> <br> <li><font color="#FF9900">Ligation of July 15 [X/P] digested <i>R21(co)-Rz21(co)</i> insert into [S/P] digested pSB1C3-<i>S21mut(co)</i></font></li></td>		
  </tr>
  <tr>
    <td>Tuesday 21</td>
    <td></td>
    <td><li>Transformation of pGOv4-Ribo_<i>S21WT(co)</i>, pGOv4-Ribo_<i>R21(co)</i> and pGOv4-Ribo_<i>Rz21(co)</i></li></td>
    <td></td>
    <td><li><font color="#0099FF"> Transformation of July 20 ligation product pSB1C3-<i>S21mut(ori)-R21(co)-Rz21(co)</i> (21Lysis_Sm(o_c_c))</font></li> <li><font color="#FF9900">Transformation of July 20 ligation product pSB1C3-<i>S21mut(co)-R21(co)-Rz21(co)</i> (21Lysis_Sm(c_c_c))</font></li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 22</td>
    <td></td>
    <td></td>
    <td><li>Redo the transformation of pGOv4-Ribo_<i>R21(co)</i></li></td>
    <td><li><font color="#0099FF"> Colony PCR on the 21Lysis_Sm(o_c_c) clones revealed that the colonies contained inserts of the wrong sizes</font></li><br><li><font color="#FF9900">Colony PCR on the 21Lysis_Sm(c_c_c) clones showed bands of the expected size</font></li><br><li><font color="#0099FF"> Redo the restriction digestion of <i>R21(co)-Rz21(co)</i> with [S/P]</li><li>Gel purification</font></li></td>		
    </tr>
  <tr>
    <td>Thursday 23</td>
    <td></td>		
    <td></td>	
    <td><li>Extraction of the plasmids pGOv4-Ribo_<i>S21WT(co)</i>, pGOv4-Ribo_<i>R21(co)</i> and pGOv4-Ribo_<i>Rz21(co)</i></li><li>Restriction digestion of Ribo_<i>S21WT(co)</i> with [E/P] & [E/S], Ribo_<i>R21(co)</i> with [E/P] & [X/P] and Ribo_<i>Rz21(co)</i> with [E/P] & [X/P]</li></td>	
    <td><li><font color="#0099FF">Redo the ligation of the [X/P] digested <i>R21(co)-Rz21(co)</i> insert into [S/P] digested pSB1C3-<i>S21mut(ori)</i></li><li>Transformation</font></li><li>Ligation of [X/P] digested BBa_I13504 (GFP) insert into [S/P] digested 21Lysis(o_o_o)</li></td>
  </tr>
  <tr>
    <td>Friday 24</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li><font color="#0099FF">Colony PCR on 21Lysis_Sm(o_c_c) clones showed bands of the expected size</font></li></td>
  </tr>
  <tr>
    <td>Saturday 25</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 11 : July 27 – August 1</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 27</td>
    <td colspan="4" bgcolor="#FFB2D1"></td>		
  </tr>
  <tr>
    <td>Tuesday 28</td>
    <td></td>
    <td></td>
    <td><li>Extraction of the plasmid pGOv4-Ribo_<i>Rz21(co)</i></li><li>Redo the restriction digestion of Ribo_<i>S21WT(co)</i> and Ribo_<i>R21(co)</i> with [E/P]</li></td>
    <td></td>		
  </tr>
  
  <tr>
    <td>Wednesday 29</td>
    <td></td>
    <td></td>
    <td><li>Ligation of the [E/P] digested Ribo_<i>S21WT(co)</i> insert into the [E/P] digested pSB1C3 vector [E/P] & transformation</li> <li>Ligation of the [E/P] digested Ribo_<i>R21(co)</i> insert into [E/P] digested pSB1C3</li><li>Extraction of the plasmids pGOv4-Ribo_<i>S21WT(co)</i>, pGOv4-Ribo_<i>R21(co)</i> and pGOv4-Ribo_<i>Rz21(co)</i></li><li>Restriction digestion of Ribo_<i>S21WT(co)</i> with [E/S], Ribo_<i>R21(co)</i> with [X/P] and Ribo_<i>Rz21(co)</i> with [E/P]</li> <li>Gel purification</li></td>
    <td><li>Redo the ligation of the [X/P] digested GFP insert into the [S/P] digested pSB1C3-21Lysis(o_o_o)</li><li>Transformation</li></td>		
    </tr>
  <tr>
    <td>Thursday 30</td>
    <td></td>		
    <td></td>	
    <td><li>Restriction digestion of Ribo_<i>R21(co)</i> with [X/P]</li></td>	
    <td><li>Colony PCR on 21Lysis(o_o_o)-GFP clones showed bands of the expected size</li></td>
  </tr>
  <tr>
    <td>Friday 31</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li><font color="#0099FF">Restriction digestion of 21Lysis_Sm(o_c_c) with [X/P]</li><li>Gel purification</font></li> <br> <li><font color="#FF9900">Redo the extraction of the plasmid pSB1C3-21Lysis_Sm(c_c_c)</li><li>Restriction digestion of 21Lysis_Sm(c_c_c) with [X/P]</li><li>Gel purification</font></li></td>
  </tr>
  <tr>
    <td>Saturday 1</td>
    <td></td>
    <td></td>
    <td></td>
    <td></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 12 : August 3 – 8</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 3</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li><font color="#127633">Extraction of plasmid pGOV4-<i>S21WT(co)</i>/li>
    <li>Restriction digestion with [E/S]</li>
    <li>Gel purification</li>
    <li>Ligation of the [E/S] digested <i>S21WT(co)</i> and July 22 [X/P] digested <i>R21(co)-Rz21(co)</i> into July 22 [E/P] digested pSB1C3</font></li> <br> 
    <li><font color="#00CC00">Extraction of the plasmid pGOv4-<i>lacIq</i> (pLacIQ_LacI_L8-UV5 lac)</li>
    <li>Restriction digestion with [E/S]</li>
    <li>Gel purification</font></li> <br> 
    <li><font color="#0099FF">Ligation of the [E/S] digested <i>lacIq</i> and [X/P] digested 21Lysis_Sm(o_c_c) into [E/P] digested pSB1C3</font></li>
    <li><font color="#FF9900">Ligation of the [E/S] digested <i>lacIq</i> promoter cassette and [X/P] digested 21Lysis_Sm(c_c_c) into [E/P] digested pSB1C3</font></li></td>		
  </tr>
  <tr>
    <td>Tuesday 4</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li>Transformation of Aug 3 ligation product</li></td>		
  </tr>
  
  <tr>
    <td>Wednesday 5</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li><font color="#127633">Colony PCR on Aug 4 transformed pSB1C3-21Lysis(c_c_c). The clones contained inserts of the expected size</font></li><br><li><font color="#0099FF">Colony PCR on Aug 4 transformed pSB1C3-lacIq-21Lysis_Sm(o_c_c). The clones contained the wrong inserts</font></li><br><li><font color="#FF9900">Colony PCR on Aug 4 transformed pSB1C3-lacIq-21Lysis_Sm(c_c_c). The clones contained the wrong inserts</font></li></td>		
    </tr>
  <tr>
    <td>Thursday 6</td>
    <td></td>		
    <td></td>	
    <td></td>	
    <td><li><font color="#127633">Restriction digestion of 21Lysis(c_c_c) with [E/X]</li>
    <li>Gel purification</li>
    <li>Ligation of the [E/S] digested lacIq into the [E/X] digested pSB1C3-21Lysis(c_c_c)</font></li>
    <li>Restriction digestion of <font color="#0099FF">21Lysis_Sm(o_c_c)</font> and <font color="#FF9900">21Lysis_Sm(c_c_c)</font> with [E/X]</li>
    <li>Gel purification</li>
    <li>Redo the ligation of the [E/S] digested lacIq into the [E/X] digested pSB1C3-<font color="#0099FF">21Lysis_Sm(o_c_c)</font> and pSB1C3-<font color="#FF9900">21Lysis_Sm(c_c_c)</font> respectively</li></td>
  </tr>
  <tr>
    <td>Friday 7</td>
    <td></td>		
    <td></td>
    <td></td>
    <td><li>Transformation of Aug 6 ligation product</li> <br> <li><font color="#127633">Redo the restriction digestion with [E/S] on the plasmid pGOv4-<i>S21WT(co)</i></li><li>Gel purification</font></li></td>
  </tr>
  <tr>
    <td>Saturday 8</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li>* Colony PCR on Aug 7 transformed cells. The clones <font color="#127633">lacIq-21Lysis(c_c_c)</font>, <font color="#0099FF">lacIq-21Lysis_Sm(o_c_c)</font> and <font color="#FF9900">lacIq-21Lysis_Sm(c_c_c)</font> all contained inserts of the expected sizes</li><br><li><font color="#127633">Ligation of the [E/S] digested <i>S21WT(co)</i> into the [E/S] digested pSB1C3</li><li>Transformation</font></li></td>
  </tr>
</table>

<table id="t01" border="1">
  <tr>
    <th colspan="5">Week 13 : August 10 – 16</th>
  </tr>
  <tr>
    <td width="20%">Date</td>
    <td width="20%">Group 1</td>		
    <td width="20%">Group 2</td>
    <td width="20%">Group 3</td>
    <td width="20%">Group 4</td>
  </tr>
  <tr>
    <td>Monday 10</td>
    <td></td>
    <td></td>
    <td></td>
    <td><li><font color="#127633">Colony PCR on Aug 8 transformed cells. The <i>S21WT(co)</i> clones contained inset of the expected size</li></td>		
  </tr>
</table>  <!-- end of lysis 21 -->


                            
			</div><!-- end container -->
		</div><!-- end main-wrap -->

		
<p id="back-top">
		<a href="#top"><span></span></a>
</p>

	<script type="text/javascript" src="files/theme/plugins.js"></script>
	<script type="text/javascript" src="files/theme/custom.js"></script>

<!--//back to top arrow-->
<script type="text/javascript">
$(document).ready(function(){

	// hide #back-top first
	$("#back-top").hide();
	
	// fade in #back-top
	$(function () {
		$(window).scroll(function () {
			if ($(this).scrollTop() > 100) {
				$('#back-top').fadeIn();
			} else {
				$('#back-top').fadeOut();
			}
		});

		// scroll body to 0px on click
		$('#back-top a').click(function () {
			$('body,html').animate({
				scrollTop: 0
			}, 800);
			return false;
		});
	});

});
</script>

	</body>
</html>

{{:Team:CityU_HK/Template/css}}
{{:Team:CityU_HK/Template/Footer}}